Collar rot of vegetables by Sclerotium rolfsii is seriously a damaging disease worldwide. Tropical and sub-tropical weather with high temperature and soil moisture favors disease severity and its spread. At least 500 species of crops are the hosts of Sclerotium rolfsii. Variations in pathogenicity, aggressiveness or virulence, geographical locations and seasonal influence exist among the isolates of the pathogen. Eight isolates of S. rolfsii were established from the collection of the pathogen from eggplant, tomato, spinach and lentil from four districts of Bangladesh. The isolates were characterized on their morphology, growth and molecular aspects. Growth rate, lesion size on the inoculated plant and plant mortality rate goverened the virulence of the isolates as determined through RAPD analysis. Isolates from tomato proved most virulent.
The importance of poygalacturonase produced by many plant pathogens has been strongly implicated in contributing to pathogenicity and virulence12About 1 U of PGase activity was defined as the amount of enzyme required to release 1 Python Crash Course + Hacking PG Wizard Books mm1 min1 galacturonic acid under standard assay conditionsThe results indicated that all tested isolates contrasted in their virulence as evidenced by the diameters of rotted areas in Weekly Cash Flow Statement Template For Small Business inoculated tomato fruitsThe PCR conditions were: 94C for 5 min, followed b y 40 cycles of 1 min at 94C for 75 sec (Depending on the type of primer used), 2 min at 72C A+3D+Virtual+Learning+Environment+Based+on+Artificial+Intelligence 10 min of final extension at 72CTwo major clusters were observed, the first cluster include isolates 1 and 2 but the rested isolates formed cluster two 07f867cfac